Methodology for the determination silmultu00c2nea of ascorbic acid and uric acid in human plasma by HPLC - UV

摘要

Method for the simultaneous determination of ascorbic acid and uric acid in human plasma by HPLC with detection by UV exclusion iu00d3nica. To obtain a correct and precise quantification, especially of ascorbic acid in plasma, it is necessary to stabilize the samples before the store.The separation cromatogru00c1fica was obtained by a eluiu00c7u00c3o isocru00c1tica, using an analytical column HEMA - Bio sb 1000 and a mobile phase buffer constituu00cdda by phosphate, pH 2.4.existem few methods on simultaneous quantification of ascorbic acid and uric acid.This methodology allows the determination of both antioxidants in the first 10 mm after the injection of the sample and is well suited to be implemented, with high yield, in routine tests and / or in clinical tests.This invenu00c7u00c3o allows, in a single separation cromatogru00c1fica, quantify the two compounds, which allows a rapid implementation, with reduction of the volumes of the sample and the solvents used, as well as of the time of use of the equipment of hpjjc.This methodology has been validated, taking into account the accuracy, repeatability, specificity, sensitivity, linearity within the ranges of concentrations referred to and recovery from, in this way, solve problems of time, availability of HPLC egastos with solvents and love Stras.