白芍总苷对糖尿病大鼠肾脏巨噬细胞浸润及增殖的影响

摘要

目的 探讨白芍总苷(TGP)对糖尿病大鼠肾脏保护作用的机制是否与抑制肾脏巨噬细胞浸润及增殖有关.方法 采用链脲佐菌素诱导大鼠糖尿病模型,随机分对照组、模型组与TGP给药组[50,100和200 mg·kg-1·d-1],8周后应用免疫组化及免疫双染技术检测各组大鼠肾组织巨噬细胞浸润、增殖情况.结果 TGP呈剂量依赖性降低糖尿病大鼠24 h尿清蛋白排泄率.模型组大鼠肾脏PCNA+细胞数、浸润巨噬细胞数及PCNA+巨噬细胞数明显高于对照组(P＜0.01);TGP组大鼠肾脏PCNA+细胞数、浸润巨噬细胞数及PCNA+巨噬细胞数明显低于模型组(P＜0.05,P＜0.01).结论 TGP可明显改善糖尿病大鼠早期肾损害,其机制可能部分与调节肾脏同有细胞增殖,抑制肾组织内巨噬细胞的浸润及增殖有关.%Objective To investigate the effects of total glucosides of paeony (TGP) on renal macrophage accumulation and proliferation in the kidney from diabetic rots. Methods Diabetic rats were induced with streptozotocin, and TGP (50, 100,200mg · kg-1 ) was administered orally once a day for 8 weeks. ED - 1 and PCNA positive cells were measured with immunohistochemistry and double immunohistochemistry. Results Elevated 24 - hours urinary albumin excretion rate was markedly attenuated by TGP treatment with 50, 100 and 200 mg· kg-1 in diabetic rats. In diabetic kidney group, the number of PCNA+ cells significantly increased compared with that in control ( P ＜ 0.01 ). Marked accumulation and proliferation of macrophages were observed in diabetic kidney ( P ＜ 0. 01 ), which significantly inhibited by treatment with TGP (P ＜0.01 ). ED- 1 and PCNA double positive cells were significantly increased in kidneys from diabetic rats.Elevated PCNA+ and ED - 1, PCNA double positive cells were significantly attenuated by TGP treatment ( P ＜ 0.01 ). Conclusion Our data suggest that TGP treatment ameliorates early renal injury via the inhibition of macrphage infiltration and proliferation, and regulation of renal cell proliferation in the kidneys from diabetic rats.