Effects of Radix Total Glucosides of Paeonia on the Endoplasmic Reticulum Stress and Macrophage Recruitment on Diabetic Cardiomyopathy Rats

摘要

Aim To investigate the effects of Total glucosides of paeonia (TGP) on on myocardial ischemia reperfusion rats and the role of Wnt/β-catenin signaling pathway was also investigated. Methods Sprague-Dawley rats were randomly divided into 5 groups: sham operation group, IR group, TGP low-dose group (i.g.50mg/kg), TGP middle-dose group (i.g.100mg/kg), TGP high-dose group (i.g.200mg/kg). The level of myocardial Wnt3a, GSK-3β(p-GSK-3β), β-catenin express were measureed by western blot. Myocardial infarction size was detected by TTC staining. The apoptotic cardiomyocytes were assessed through TUNEL. Results Compared with IR group, TGP can increase LVSP, LVEDP, +dp/dtmax, LVEF. The expressions of Wnt3a, GSK-3β(p-GSK-3β),β-catenin, the apoptotic index and myocardial infarction size were lower in the TGP group than those in I/R group (P < 0. 01). Conclusion The protective effect of TGP on myocardial ischemia reperfusion rats is obviously, Wnt/β-catenin signaling pathway maybe involved in the mechanism.