Flow Perfusion Culture Induces The Osteoblastic Differentiation Of Marrow Stromal Cell-scaffold Constructs In The Absence Of Dexamethasone

摘要

We observed that titanium fiber mesh scaffold/marrow stromal cell constructs grown under flow perfusion were more mineralized than their static counterparts, even in the absence of dex. However, there was a synergistic effect between flow perfusion and presence of dex in the culture medium, resulting in abundant mineralized matrix after 16 days of culture. There was also an indication that osteogenic differentiation might be enhanced under flow perfusion by exposure to dex only during the initial cell expansion phase. Finally, no osteogenic differentiation was observed for scaffold/cell constructs when exposed to neither dex nor flow perfusion.