Methods for inducing differentiation between bone marrow stromal cells and skeletal muscle cells, between bone marrow stromal cells and myoblasts, and between myoblasts and skeletal muscle cells

摘要

METHODS FOR INDUCING DIFFERENTIATION BETWEEN BONE MARROUS STROMAL CELLS AND SKELETAL MUSCULAR CELLS, BETWEEN BONE MARROUS STROMAL CELLS, AND BETWEEN MYBLASTUS AND CELLULAR CELLS. A method of inducing differentiation between a bone marrow stromal cell and a skeletal muscle cell effectively, simply and reproducibly, comprising the steps of: (a) adding one or more substances, selected from the group consisting of an AMP elevating operating agent cyclic, a cyclic AMP analog and a cell differentiation survival working factor, to a bone marrow stromal cell culture (note that the bone marrow stromal cell is not treated with a demethylating agent) and perform cultivation; (b) obtain a myoblast culture by introducing the Notch gene and / or a Notch signaling related gene into the cell obtained in step (a) and culturing (note that the cultivation does not include co-cultivation a cell into which a gene was introduced and a cell into which a gene was not introduced); and (c) adding a Notch ligand to the myoblast culture obtained in step (b) and culturing.