Bio-Electrochemical Analysis of L-Ascorbic Acid Concentration in between Glaucoma/Cataract Patients Aqueous Humor

摘要

Glaucoma is the second leading causes of vision loss in the world after cataract and irreversible, particularly among the elderly, several years past, most of the molecular mechanisms leading to primary open angle glaucoma (POAG) are still unknown, however scientists discovered ascorbic acid (AA) concentration is reduced in cataract and glaucomatous patients aqueous humor compared with healthy eyes. For understand relationship between AA concentration and glaucoma, we try to develop a simple, sensitive and accurate sensor device for detection in AA. Electrochemical sensor featured with short analytical time, low cost, high sensitivity compared with other methods, in this work, a new electrochemical aptamer sensor for detecting AA was fabricated based on AA specific aptamer linked with polydopmine (PD) layer and immobilized on screen printed carbon electrode (SPCE). Cyclic voltammetry (CV) was used to investigate AA concentration dropped on modified electrode. Both analyze real human aqueous humor sample or commercial AA solution, aptamer electrode performed a good accuracy compared with high performance liquid chromatography (HPLC).