Study of Biochemical IC Chip-set (15th report) - Development of Biochemical IC chip for homogenization of cells and tissues

摘要

The world's first micro ultrasonic homogenizer chip, a new type of biochemical IC chip, is proposed and developed successfully. The homogenizer chip utilizes ultrasonic cavitation to disrupt cells. Both a piezoelectric element and an exponential horn to generate ultrasonic cavitation are hybridized in the chip by using our original microstereolithography technique named "hybrid IH process". We succeeded in disrupting various cells within only 3 seconds by using the chip. Moreover, we succeeded in disrupting the internal-organs organization of a mouse within only 5 seconds. The efficiency of homogenization was almost 100%. The micro homogenizer chip is useful for pretreatment of various biochemical and biological analysis in microscale.