Determination of Hydroxymethylfurfural (HMF) in Honey Based on p-Toluidine as a Reagent Using Spectrophotometric Method

摘要

Spectrophotometric determination of hydroxymethylfurfural (HMF) based on p-toluidine as a reagent has been studied. The absorption intensities were measured at a wavelength range of 250 to 500 nm to record the absorption spectra before and after reaction with the HMF. The solution response was obtained at pH 3. The concentration of HMF was 5 × 10~(-3) M, whereas for p-toluidine was 8 × 10~(-3) M. The relative standard deviation (R.S.D.) of reproducibility was 3.33% when concentration of p-toluidine 3 × 10~(-3) M was used. The photostability was found to be good with 1.9%.