Fermentation Production of Succinic Acid from Sucrose by Engineered Escherichia coli JH208

摘要

Currently, succinic acid produced from microbial fermentation has attracted great interests by many researchers, due to its low cost and friendly environmental benefits. Among various microbe, Escherichia coli species have been considered as suitable succinic acid-producing strains, which could utilized a wide range of substrate. In order to acquire sucrose-consumed strains, a genetically modified E. coli JH208 was constructed using the RED recombination system and the flipase recognition target (FRT) site-specific recombinant technology. In 48 h, the production of succinic acid from sucrose by JH208 is 48.46 g/L, the productivity is 1.01 g L~(-1) h~(-1), with a succinic acid yield of 0.83 g/g sucrose. The by-products were very low in the fermentation, acetic acid was 4.22 g/L, the ethanol concentration was less than 0.10 g/L, and formic acid and ethanol was not detected.