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METHOD FOR PREPARING RECOMBINANT ESCHERICHIA COLI TO PRODUCE SUCCINIC ACID THROUGH FERMENTATION

机译：发酵生产重组>埃希氏菌的方法

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摘要

The invention relates to a method for preparing recombinant Escherichia coli to produce succinic acid through fermentation, belonging to the technical field of bioengineering. The invention is mainly for increasing the growth rate of the strain under the anaerobic condition and the synthesis rate of succinic acid by overexpressing NAD+ biosynthesis-related genes in Escherichia coli, and adding a certain concentration of nicotinic acid to the anaerobic culture medium as the precursor material of NAD+ biosynthesis,. By this method, the problem of low production rate of the succinic acid during specific anaerobic fermentation of the recombinant Escherichia coli can be solved, at the same time, a means is provided for anaerobic production of an enzyme preparation by using the Escherichia coli.

机译：本发明涉及一种通过发酵制备用于生产琥珀酸的重组大肠杆菌的方法，属于生物工程技术领域。本发明主要是通过在大肠杆菌中过表达NAD +生物合成相关基因，并向其中添加一定浓度的烟酸来提高厌氧条件下菌株的生长速度和琥珀酸的合成速度。作为NAD +生物合成的前体材料的厌氧培养基。通过这种方法，可以解决重组大肠杆菌的特定厌氧发酵过程中琥珀酸生产率低的问题，同时提供了一种厌氧生产酶制剂的方法。通过使用大肠杆菌。 展开▼

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公开/公告号WO2012119546A2

专利类型

公开/公告日2012-09-13

原文格式PDF

申请/专利权人 NANJING UNIVERSITY OF TECHNOLOGY;JIANG MIN;MA JIANGFENG;CHEN KEQUAN;GUO TING;WEI PING;OUYANG PINGKAI;
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申请/专利号WO2012CN72012

发明设计人 JIANG MIN;MA JIANGFENG;CHEN KEQUAN;GUO TING;WEI PING;OUYANG PINGKAI;
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申请日2012-03-06

分类号none;

国家 WO

入库时间 2022-08-21 17:13:55

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3. METHOD FOR PREPARING RECOMBINANT ESCHERICHIA COLI TO PRODUCE SUCCINIC ACID THROUGH FERMENTATION [P] . 世界知识产权组织专利： WO2012119546A3 . 2012-11-01

机译：发酵生产重组>埃希氏菌的方法

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机译：糖原生产者的重组细菌菌株（版本）和使用该菌株制备糖原的方法

5. Method prepares L-threonine production 472 t-23 97 of strain Escherichia coli by fermentation and using this: relying partially on isoleucine when preparing 472 T-23 of L-threonine-producing strain Escherichia coli using therefore fermentation method, resistance with the gene and threonine operon that carry in plasmid is to penicillin, it is possible that generating in medium and isoleucine concentration boundary, appropriately combined the 3 to 1. of guarantee0 gram per liter of corn is precipitous, molass21:02570-91 11:279975 [P] . CZ256991A3 . 1993-03-17

机译：该方法通过发酵制备大肠杆菌的L-苏氨酸产量472 t-23 97，并利用该方法：使用发酵法制备产L-苏氨酸的大肠杆菌472 T-23时部分依赖异亮氨酸，具有该基因的抗性和携带质粒的苏氨酸操纵子对青霉素，有可能在培养基和异亮氨酸的浓度范围内产生，适当地将每升玉米中0克3比1保证混合，molass21：02570-91 11：279975

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9. A Novel Fermentation Pathway in an Escherichia coli Mutant Producing Succinic Acid, Acetic Acid, and Ethanol [C] . MARK I. DONNELLY, CYNTHIA SANVILLE MILLARD, DAVID P. CLARK, Biotechnology for Fuels and Chemicals . 1998

10. Separation of succinic acid from fermentation broths and esterification by a reactive distillation method [D] . Orjuela Londono, Alvaro 2010

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