P59: COMPARATIVE ANALYSIS OF FATTY ACIDS COMPOSITION OF VEGETABLE OILS AND ANIMAL FATS BY APPLICATION OF GC-MS AND NMR TECHNIQUES

摘要

The purpose of this paper was to compare the composition (weight% of total identified FA) in saturated (SFA), monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids from 3 food matrices (sunflower oil, palm oil and lard) by 2 different techniques, GC-MS and NMR. FAMEs analysis from food samples was performed using a gas chromatograph coupled with a Triple quadrupole mass spectrometer. NMR spectra were recorded on a Bruker Advance 400 MHz spectrometer, operating at 9.4 Tesla corresponding to the resonance frequency of 400.13 MHz for the ~1H nucleus. The composition in fatty acids determined by these two techniques was compared and the difference between the mean values determined by GC-MS and NMR was not more than ± 15% for sunflower oil, and lard, and ± 6% for palm oil.