Analytical Approaches for Characterization of Teeth Whitening Gels Based on Natural Extracts

摘要

The present study reports the analytical approaches for characterization of teeth whitening gels based on natural extracts of fruits and medicinal plant. High performance liquid chromatography (HPLC) with UV detection were used for identification and quantification of (oxalic, citric, tartaric, malic, succinic and fumaric acids), flavonoids (catechin, epicatechin, rutin and quercetin) and phenolic acids (vanillic acid, p-coumaric acid, ferullic acid) from studied gels samples used for teeth whitening. The organic acids were used as active agent with action in tooth discoloration and stain removal. Organic acids were separated through a Carbosep Coregel 87H3 column at 35°C and detection at 214 nm. The mobile phase was the sulphuric acid 0.005 M solution. The total quantity of organic acids of these gel samples was determined. The action of flavonoids have controlled dental caries and inhibited plaque formation. Separation of flavonoids and phenolic acids was carried out on Lichrosorb RP-C18 column at 40 °C and detection at 290 nm. The mobile phase was a mixture of methanol and solution 0.1% formic acid in a gradient elution. The flavonoids, catechin, epicatechin, rutin, quercetin, and phenolic acids, vanillic acid,p-coumaric acid, ferullic acid, were determined quantitative in the teeth whitening gels on natural extracts of fruits and medicinal plant.