Bioinformatics Analysis of the Gene CYP83B1 in Cabbage (Brassica oleracea var. capitata)

摘要

CYP83B1 is an important glucosyltransferase enzyme in glucosinolate biosynthesis. Here, the Brassica oleracea var. capitata CYP83B1 (BocCYP83B1) gene sequence was obtained from Brassica database (BRAD), and preformed for bioinformatics analysis. The BocCYP83B1 gene mapped to chromosomes 8, and contains an open reading frame of 1,473 bp that encodes a 490-amino acid protein with a calculated molecular mass of 55.89 kD and an isoelectric point (pI) of 8.89. Subcellular localization predicted the BocCYP83B1 gene was in the chloroplast. The conserved domain of the BocCYP83B1 protein is belonged the p450 superfamily. The CYP83B1 protein is most closely related to B. rapa. The findings of the present study provide a molecular basis for the elucidation of CYP83B1 gene function in cabbage.