The research of subcellular localization of ethylene response factor LeERFl in tomato fruit

摘要

Ethylene is an endogenous plant hormone that influences many aspects of plant growth and development. Ethylene responses is conducted by ethylene signal transduction pathway. Ethylene Responsive Element Binding Proteins (EREBPs) are transcriptional factors function in the ethylene signaling pathway in plants, helping to regulate ethylene responses by regulating genes transcription and expression. We have cloned Tomato LeERFl gene which was identified as members of the EREBPs family, characterized by a conserved ERF domain. In this research, in order to further examine the location of the LeERFl, immunocytochemical localization in vascular tissues of tomato fruit was proformed. Our results suggested LeERFl was mainly localized in the nucleus (N)> nucleolus (Nu) and plastid (P), and little LeERFl signal was detected in the cell wall (CW) and vaculoe (V) . During the phase of immature green stage (IG) of tomato fruit, there were a few LeERFl protein in the cells of tomato tissues. As the development, the signal of LeERFl increased obviously in the nucleus (N), nucleolus (Nu) and plastid (P). And there were larger LeERFl protein in breaking tomato fruit than in green tomato fruit identified by Western blotting. The experiments showed that LeERFl had relationship with the ripening of tomato fruit.