Regulatory Factor Optimization by Response Surface Methodology for Biogas Yield by Methanobacterium Soehngenii Strain YH

摘要

The methanogen medium, supplemented with malate, succinate and glutamate, was used as substrates for high biogas yield by M. soehngenii YH. The fermentation medium was optimized by response surface methodology. First, more compatible concentrations of malate, succinate and glutamate were ensured. In the second step, the concentrations of the three supplemental nutrients above were further optimized using a Box-Behnken design. The average biogas yield (450 mL), 61% higher than those of the control in five independent samples, was obtained on the laboratory scale with optimized initial additions of malate, succinate and glutamate corresponding to 0.52 g/L, 1.06 g/L and 0.33 g/L, respectively. These would lay a foundation for increasing the efficiency of biogas synthesis and exploring a late-model culture technics of M. soehngenii.