Express Instrumental Diagnostics of Diseases Caused by Retroviral Infections

摘要

A surface plasmon resonance (SPR) based immune biosensor for rapid diagnostics of bovine leucosis (BL) caused by retroviruses, is described. BL was detected through the specific antibody (Ab) to the antigen (Ag) of the virus in blood and milk serum. Sensitivity of the analysis achieved by this biosensor is similar to that of the ELISA method and much higher than the radial immunodiffusion test. The optimal dilutions of the blood and milk serum were 1:500 and 1:20 respectively. The turnaround time of the analysis was less than 30 min including the time for the Ag immobilization on the transducer surface, blocking free binding sites, Ag-Ab interaction and all washing procedures. This time may be further shortened to 5 min if the transducer surface is prepared in advance. Pre-treatment of the gold surface with dodecanethiol increases sensitivity of the analysis in comparison with the untreated surface. In contrast to the ELISA method, the immune biosensor technique allows analysis without additional labelled molecules.