NSE (Neurone Specific Enolase) expression of the different time points in PC12 cells to differentiate into nerve cells

摘要

NSE plays a vital role in PC12 cell differentiation, expecialy the length of the synapse and the formation of microtubules. Here, NGF (Nerve Growth Factor) and OGD (oxygen-glucose deprivation) were used to stimulate PC12 cells, and convert them into neurons to establish an ischemia in vitro model. In order to further find NSE expression in PC12 cells. Western blot were used to determinate the protein expression of NSE. NSE expression was determined by western blotting after different treatment time periods. The results shown are representative of three repeated experiments. The expression was different with different time. Consult the relevant literature, rats or cell tumor cells adrenal collaterals (PC12 cells) can be NGF induction into Nerve cells, NSE exists in PC12 cell differentiation process, it and the length of the synapses and microtubules formation of closely related, the NSE in synaptic neuron growth plays an important role.