Detection method of gene expression of molecular markers characterizing chondrogenic differentiation of stem cells specific for animal model of a horse, panel of primers for making the method and use of such a primer panel

摘要

ufeffpanel primers and method for detection of gene expression of molecular markers of differentiation specific for animal model chondrogennu00ed characterising the horsewherein with isolated dna from specifically differentiated horse mesenchymu00e1lnu00edch cells into chondrocytu016f (cells, cartilage) by polymerase chain reaction (pcr) using primers covering the panel, at least one of the oligonukleotidovu00fdch primerovu00fdch pairs: acan f: 5 '- gct ccg acc gat act ctc tg - 3 (20 mer), acan r: 5 '- ggc tca caa tgg ggt atc tg - 3 (20 mer)for the detection of gene sequences aggrecanu (acan); col1a1 f: 5 '- very quiet ccc tao ggg tga cag ag - 3 (20 mer), col1a1 r: 5' - very quiet very quiet acc acc gct agt tt - 3 (20 mer) for the detection of gene sequences of type i collagen alpha 1 (col1a1); col2a1 f: 5 '- aag cga aag ttg gtc cct ct, 3 (20 mer), col2a1 r: 5 '- gtc cct ttt cac cag ctt tg - 3 (20 mer) for the detection of gene sequences of collagen type iithe alpha 1 (col2a1); sox9 f: 5 '- tca agc aag aac aag cct ca 3 (20 mer), sox9 r: 5' - gcg gct ggt act tgt agt cc, 3 (20 mer) for the detection of gene sequences transkripu010dnu00edho factor sox0 (sox9); vcan: 5 '- gcc caa aat ggg aat atc aa, 3 (20 mer), vcan: 5' - cgc tcg gta gtg cac aa aaa - 3 (20 mer) for the detection of gene sequences versicanu (vcan) and subsequently by electrophoresis pcr products of reaction.this solution makes it possible to quickly, simply and specifically detect cells differentiated into chondrocytu016f using specific markers in cell isolates, specially in the animal species of horse.