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PCR primer set for bacterial DNA amplification, bacterial species detection and / or identification kit, and bacterial species detection and / or identification method

机译:用于细菌DNA扩增,细菌种类检测和/或鉴定试剂盒以及细菌种类检测和/或鉴定方法的PCR引物组

摘要

An object of the present invention is to provide a primer set useful for further improvement of sensitivity in detection or identification of bacterial species in a sample by a PCR method, a kit for PCR using the primer set and a method of detection or identification of bacterial species in a sample using the primer set, as well as to further improve sensitivity in detection or identification of bacterial species in a sample by conducting a PCR method using the primer set with a minimized contamination amount of bacterial nucleic acid. A primer set according to the present invention comprises at least one primer pair in seven primer pairs obtained by selecting one primer pair from each of Groups S1 to S7 consisting of specific primers in order to achieve the above objects. In addition, a kit for detection of bacterial species according to the present invention comprises at least one primer pair in seven primer pairs obtained by selecting one primer pair from each of Groups K1 to K7 consisting of specific primers.
机译:本发明的目的是提供一种引物组,用于进一步提高通过PCR方法检测或鉴定样品中细菌种类的敏感性的引物组,用于使用该引物组进行PCR的试剂盒以及检测或鉴定细菌的方法。使用引物组的样品中的细菌物种,以及通过使用引物组进行PCR方法并最小化细菌核酸污染量的PCR方法,进一步提高检测或鉴定样品中细菌种的敏感性。为了实现上述目的,根据本发明的引物组在七个引物对中包含至少一个引物对,所述七个引物对是通过从由特定引物组成的S1至S7组的每一个中选择一个引物对而获得的。另外,根据本发明的用于检测细菌种类的试剂盒包括在七个引物对中的至少一个引物对,所述七个引物对是通过从由特异性引物组成的组K1至K7的每一个中选择一个引物对而获得的。

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