Proteomics of Alzheimer's disease: Candidate proteins identified in serum as diagnostic biomarker for Alzheimer's disease

摘要

Alzheimer's disease(AD) is the most common cause of dementia of late life. To enhance our understanding of AD proteome, the serum proteins were analyzed using two-dimensional gel electrophoresis combined with nano-high performance liquid chromatography electrospray ionization tandem mass spectrometry followed by peptide fragmentation patterning. In this study, six protein spots with differential expression were identified. Five up-regulated proteins were identified asactin, apolipoproteinA-IV, inter-alpha-trypsin inhibitor heavy chain H4, alpha-1-antitrypsin, andantithrombin-III; one protein, activity-dependentneuroprotector homeoboxprotein (ADNP) was down-regulated in AD patients. These proteins with differential expression in theserum may serve as potential indicators of AD. Our results suggested that ADNP may play an important role in slowing the progression of clinical symptoms of AD.