Determination of Endogenous Steroids in Human Blank Serum using a Triple Quadrupole Mass Spectrometer with Ion Funnel Technology in Positive/Negative Modes

摘要

Baseline separation of thirteen steroids with the exception of estradiol is achieved under 8.5 minutes. However, estradiol is not isobaric to androsteronedione or estrone, so the quantitation calculation is not effected. The calibration curves show excellent linearity (> 0.995) with greater than three orders of dynamic range. Great accuracy, precision, reproducibility, and signal stability of LC-MS/MS (QQQ) analyses were observed for the 13 steroids. This fast and simple LC-MS/MS method is suitable for analyzing several endogenous steroids in biological matrices in a single run.