A multiplexed integrated a-Si:H photosensor for simultaneous detection of mycotoxins for point-of-use food safety applications

摘要

Food safety can be seriously compromised by the presence of mycotoxins. Their detection becomes increasingly relevant as they are subject to strict regulations in the European Union. To cope with this demand, a novel device with manually operated magnetic valves was developed and applied in a direct competitive immunoassay with multiplexed chemiluminescence detection of ochratoxin A, aflatoxin B1 and deoxynivalenol. The optical detection of chemiluminescence from the enzymatically labelled antibodies is performed with a micro fabricated array of 25 μm × 25 μm hydrogenated amorphous silicon (a-Si:H) photoconductors. This strategy provides a simple multiplexing device for mycotoxin screening on the field.