首页>
外国专利>
TIME-RESOLVED FLUORESCENCE IMMUNOCHROMATOGRAPHIC KIT FOR SIMULTANEOUS DETECTION OF MIXED CONTAMINATION OF FIVE MYCOTOXINS SUCH AS AFLATOXIN B1 AND APPLICATION THEREOF
TIME-RESOLVED FLUORESCENCE IMMUNOCHROMATOGRAPHIC KIT FOR SIMULTANEOUS DETECTION OF MIXED CONTAMINATION OF FIVE MYCOTOXINS SUCH AS AFLATOXIN B1 AND APPLICATION THEREOF
展开▼
机译:用于同时检测五种霉菌毒素的混合污染,例如黄曲霉毒素B1及其应用的时间分辨荧光免疫色谱试剂盒
展开▼
页面导航
摘要
著录项
相似文献
摘要
A time-resolved fluorescence immunochromatographic kit for simultaneous detection of mixed contamination of five mycotoxins such as aflatoxin B1 and an application thereof are disclosed. The kit comprises a time-resolved fluorescent immunochromatographic test strip and sample reaction vials each containing a europium-labeled monoclonal antibody lyophilized product; wherein the fluorescent test strip comprises a PVC substrate, and a surface of the PVC substrate is adhered with a water absorbing pad (1), a detection pad (2) and a sample pad (3) from top to bottom, adjacent pads being connected and overlapping at connections. The detection pad (2) adopts a nitrocellulose membrane as the base thereof and is arranged with a lateral quality control line (5) and five detection lines (5, 6, 7, 8, 9) from top to bottom each covered by a bovine serum albumin conjugate of each toxin. The fumonisin B1 monoclonal antibody is generated by the hybridoma cell strain Fm7A11 having China Center for Type Culture Collection (CCTCC) accession number C201636. The kit is applicable to simultaneous detection of mixed contamination of aflatoxin B1, fumonisin B1, ochratoxin A, zearalenone and sterigmatocystin.
展开▼