Macroporous membrane adsorbers with tailored affinity for proteins and bacteria via controlled 'grafting-from' of glycopolymer layers

摘要

Separations with membrane adsorbers are a very attractive and rapidly growing field of application for functional macroporous membranes [1]. Aims of further development are membrane adsorbers with higher dynamic binding capacity and with higher affinity and selectivity for certain target substances, especially based on robust chemical ligand architectures. Previously, we had reported about the analysis of correlations between pore structure and protein binding in commercial ion-exchange membrane adsorbers by using advanced microscopy [2], and about the preparation of ion-exchange membrane adsorbers with improved dynamic binding capacity via surface-selective photo-grafting of polymers layers with adjusted density of ion- exchange groups and layer cross-linking [3]. We had also demonstrated that protein-selective membrane adsorbers can be obtained by grafted polymer layers from monomers with relatively simple synthetic motives for selective supramolecular association with certain amino acids and allowing cooperative multiple binding of a protein molecule [4].