DEFINITIVE DRUG AND METABOLITE SCREENING IN URINE BY UPLC-MS/MS USING A NOVEL CALIBRATION TECHNIQUE

摘要

A UPLC-MS/MS assay has been developed and validated for multi-analyte screening in urine and provides an alternative to presumptive immunoassay screening. The assay employs a novel calibration technique to achieve normalization of matrix effect, without the use of internal standards, and provides threshold accurate screening for drugs and metabolites. Future activities will focus on automation of the TAC procedure.