Chlorinated Dioxins, Furans and Biphenyls Analysis in Complex Matrices Using Automated Extraction and Reduced Solvent Volume Column Chromatography

摘要

We analyzed PCDD/Fs and PCBs in peanut butter, top soil and fish oil. The recoveries of the ~(13)C labeled isotope dilution standards are shown (Tabel 1). The peanut butter and top soil were first extracted with Pressurized Liquid Extraction and then processed on the automated column clean up system. Recoveries found were excellent. The work showed that all target PCBs (WHO-12 plus pcb-170 and -180) were collected efficiently in the separate fraction. Reducing the conditioning and wetting steps from the original program proved to have no significant effect on the recoveries. The new reduced solvent method has also been used for the combination of classical (regular size) abn - alumina - carbon columns with a total volume of ～ 250 mLs. When HC-ABN columns are used, total solvent volume is 400 mLs. The reduced solvent volume approach to automated sample clean up has been demonstrated to be very effective, both in terms of reduced labor costs (shorter sample prep time) and reduced solvent use, and is also giving excellent recoveries with difficult matrices. This demonstrates once again the importance of automation in today's laboratory work.