Analysis of surface charge influences in interactions of nanoparticles with human bronchoalveolar lavage fluid using HPLC - MS/MS

摘要

Due to growing utilization of nanoparticles (NPs) in everyday life, it is essential to study the biological effects of these materials to allow adequate safety assessment. When NPs are in contact with a biological medium, such as fetal calf serum (FCS) or human bronchoalveolar lavage fluid (hBALf), they are immediately covered by the proteins. Over time and depending on the affinity of the proteins binding, a so-called hard or soft protein corona (HC and SC) is created. The particle characteristics and the immunological responses may be influenced by this attachment of proteins [M. Mahmoudi et al, Chem. Rev. 2011, 111, 5610-5637]. The aim of this study is to analyse the interactions of NPs with proteins found in FCS, and with proteins found within hBALf, which will allow a better understanding of how NPs behave within well established in vitro cell systems, also an insight into how NPs would behave upon inhalation into the human lung. This will provide knowledge for synthesis of safe, functional particles. The study involves a profiling and characterization of the proteins which attach to particles, and relating this interaction to specific NP characteristics. We also want to perform a differential proteome analysis to study immunological responses of adenocarcinomic lung epithelial cells (A549) treated with FCS or hBALf coated NPs.