Extraction of Mycophenolic Acid and Mycophenolic Acid Metabolite from Serum Using Supported Liquid Extraction (ISOLUTE SLE+) Prior to LCMS-MS Analysis

摘要

ISOLUTE SLE+ is a fast sample preparation alternative for biological fluids. The extraction of the immunosuppressant mycophenolic acid and its metabolite mycophenolic acid glucoronide was demonstrated using supported liquid extraction (ISOLUTE SLE+) as a viable alternative to protein crash. Improved recoveries were observed using supported liquid extraction which can ultimately lead to better quantitation and better sensitivities at lower limits of detection. Future work will include determining lower limits of detection for the analytes using ISOLUTE SLE+