Probing Native Structures Of Homologous Large Proteins With Differential Covalent Labeling and Mass Spectrometry Characterization

摘要

Modification to the protein native folds can provide structural information. Side chain labeling experiments rely on protein structure dynamics, the amino acid reactivity, solvent accessible surface area (SASA) and the micro-environment of the residue. A single labeling experiment can be both selective (like chemical labeling) and non-selective (like oxidative labeling). Combination of different labeling techniques are more likely to provide SASA information of amino acid residues (Figure 1).