Determination of HIV-1 Subtypes (A-D, F, G, CRF01_AE) by PCR with Novel Designed Primers

摘要

The specific primers were designed for each subtypes in the gp41 region. The result of PCR was compared with the subtypes which was determined by v3 sequence. The results of subtyping by PCR using the newly designed primers could detect 87% (26/30) and all of them matched with those obtained by nucleotide sequencing of the env v3 region. These primers could be used to differentiate subtypes A, B, C, D, F, G, and CRF01_-AE. This method could be applied, with several advantages, to epidemiological studies in developing countries, which face a large number of people living with HIV/AIDS.