DIFFERENT EXPRESSION OF GAP JUNCTIONAL PROTEIN CONNEXIN43 IN TWO STRAINS OF MICE AFTER ONE-MONTH IMPLANTATION OF POLY-L-LACTIC ACID

摘要

The implantation of a biomaterial often induces host inflammatory responses. Some adverse effects by the biomaterials, such as poly-L-lactic acid (PLLA) and polyurethanes (PUs) were reported in animal experiments. PLLA produced tumorigenicity in ratsafter long-term implantation. The purpose of this study was to determine the in vitro effect of PLAO3 (high-molecular weights of PLLA) and PUS (PTMO/MDI/BD) on the function of the normal human dermal fibroblasts (NHDF) and the in vivo effect of PLAO3 onthe function of the cells originated from the subcutaneous tissue in the two female mouse strains, BALB/cJ and SJL/J. The results with Scrape-loading and dye transfer (SLOT) assay, Western Blot and RT-PCR analysis clearly demonstrated that gap-junctionalintercellular communication (GJIC) and the expression of Cx43 were significantly suppressed in PLAO3-implanted group of BALB/cJ mice in compared to the control mice. While, no significant difference was found in GJIC and the expression of mRNA level buta little bit difference was observed in the Cx43 protein expression between the SJL/J implanted and the control mice. We considered that the PLAO3 suppressed irreversibly gap junctional protein connexin43 at the earlier stage after implantation and thesuppression of connexin43 gene-expression might play a vital role in the inhibition of GJIC and thus promotes the tumorigenesis.