Evaluation of genetic diversity among Italian 'farro' (T. dicoccum) populations using AFLP markers.

摘要

In the present study, ten Italian populations of "farro" (T. dicoccum), collected from their traditional cultivation areas, were analyzed with amplified fragment length polymorphism (AFLP) markers in order to evaluate their level of genetic diversity.Eight primer combinations were used for selective amplification yielding 146 polymorphic AFLPs. On average, each AFLP assay revealed a percentage of 19.1% polymorphic bands. Cluster and principal component analysis clearly showed a good grouping of thegenotypes in each single population. The correlation values between similarity estimates computed for each AFLP assay and the whole set of primers identified a number of highly informative AFLPs suggesting that AFLP analysis can be useful for an effective characterization of T. dicoccum populations.