Transgenic tobacco expressing the trichosanthin shows enhanced resistance to fungal pathogen Alternaria longipes infection

摘要

In recent years, some research suggested that ribosome inactivating proteins (RIPs) may have functions against fungal attack and maybe play a role in plant defense mechanisms. The transgenic plants expressing TCS gene have been demonstrated to possess the resistance activities against virus infection. TCS expressed in E. coli showed broad-spectrum anti-fungal activities to 9 different fungal pathogen including Alternaria longipes in vitro, and the inhibition of A. longipes rate was 16.5%. When TCS combined with chitinase or glucanase or both, the inhibition rate of A. longipes was increased to 43.2% approx 61.3%. The results suggested that TCS is a candidate to prevent plants from infection of fungal pathogen.