A Semi-Analytical Simulation and Test for Three- Dimensional Grid Array Dielectrophoresis Cells Cage

摘要

The dielectrophoresis is suitable for biomedical application especially in cell manipulation and detection. The three-dimensional grid array device is composed of two glass plates on which parallel gold thin film electrodes are fabricated, then assembling the two glass plates by electrode side face to each other. The distance between the two plates was determined by the thickness of PDMS layer, which also as a fluidic channel layer. We have proposed a semi-analytical method that complete electric and temperature field simulation of the grid structure based on asymptotic analysis, in which a dielectrophoresis potential cage was constructed for cell manipulation. It has been demonstrated with polystyrene microspheres and A549 lung cancer cells that they can be trapped into the cage. The method improves the simulation accuracy and reduces computational complexity. The impedance of microspheres and cells also tested that device gives a cell manipulation and label free cell detection integrated technology.