ANALYSIS OF INTERLEUKIN-6-INDUCED SIGNALING IN MURINE HYBRIDOMA CELL LINES

摘要

Interleukin-6 (IL-6) is a multifunctional cytokine that plays important roles in the immune response, inflammation, differentiation, and growth control in various cells. It is also reportedly involved in a variety of diseases. We found that a murine hybridoma cell line, 2E3, derived from P3U1 myeloma cells and its subclone, 2E3-O, adapted to serum-free medium differed in growth responses to IL-6. When treated with IL-6, the growth of 2E3 cells was accelerated, while that of 2E3-O cells was significantly suppressed. Because studying differences between the two cell lines could yield important insights into IL-6-induced signaling, we comprehensively analyzed the protein expression profiles of both cell lines and detected several unique spots in 2E3-Ocells.