目的:以白细胞介素1β(IL-1β)和胶质源性神经营养因子(GDNF)为诱导剂,探索小鼠胚胎中脑神经干细胞(M-NSCs)向多巴胺能神经元的的分化.为M-NSCs移植治疗帕金森病(PD)提供实验依据.方法: 在有血清条件下体外培养鼠胚M-NSCs,予以IL-1β和GDNF作诱导分化.酪氨酸羟化酶(TH)免疫细胞化学鉴定.流式细胞术检测TH阳性神经元的百分率.结果: GDNF组促进M-NSCs分化为TH阳性神经元的比例为13.53%,IL-1β组为9.66%,GDNF+IL-1β联合培养组为16.22%,空白对照组仅3.46%,差异有统计学意义.结论: IL-1β和GDNF可明显促进M-NSCs分化成多巴胺能神经元.%Objective: To explore mouse embryonic neural stem cells to dopaminergic neurons division induced by IL-1β and GDNF, and provide the experimental basis for the mesencephalic neural stem cells (M-NSCs) transplantation for treatment of Parkinson's disease. Methods: M-NSCs were cultured and proliferated, and then IL-1β and GDNF were used to induce differentiation in the condition of fetal bovine serum. Immunocytochemical techniques were used to detect the TH-ir cells from M-NSCs differentiation, and Flow cytometry was used to detect the percentage of TH-ir in all the cells. Results: The proportions of M-NSCs differentiation into TH positive cells were as follows: 13. 53％ in the GDNF group, 9. 66％ in the IL-1β group, 16.22％ in the GDNF+IL-1β group. The data of each group were much higher than those in the blank control group (3. 46 ％). The proportions of NSCs differentiation into TH positive cells showed significant difference among experimental groups. Conclusion: IL-1β and GDNF can significantly increase the differentiation of dopaminergic neurons.
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