In-situ characterization by FT-Raman spectroscopy of compounds separated on a thin layer chromatography plate

摘要

FT-Raman spectroscopy has been shown to be useful as a method of identifying compounds separated on a tlc plate. Commercial tlc plates, with or without UV fluorescent indicators, were found to be suitable for the technique provided that they had a metallic backing. Plates with polymer backing were unsuitable, as polymer bands could be seen in the spectrum. The method was demonstrated using a range of aromatic and aliphatic organic compounds.