Electrical Stimulation to Vagina Muscle cells and Rabbit bladder Muscle and Epithelial cells In-Vitro to change Growth Factor

摘要

We stimulated electrically (In-Vitro) Vagina muscle cells and Rabbit bladder muscle and Epithelial cells, in order to see the effects on growth rate in the cultures. In two medium cultures "A" (control) and "B" (stimulated) we obtained a significant difference cell growth stimulating with a bipolar wavelet with 400 mVpp and 200 Hz of frequency applied on vagina muscle cells in passing 3, and a 150 mVpp wavelet (with 3.33 Hz, and 10 Hz) on rabbit bladder muscle and epithelial cells for primary culture, passing 2 and 3. We present the results generated by stimulating on muscle and epithelial cells cultures to obtain different viability and proliferation in comparison to control cells. These results (and future ones) will be used to find an optimal wavelet to control the growth factor in cells to improve the quality of neo-tissues in human bladder.