Biosynthesis of Immunostimulatory Compounds from Ganoderma sp. from European Habitats by Submerged and Solid State Cultivation

摘要

An original strain of Ganoderma lucidum MZKI G97, was isolated from Slovenian forests was cultivated in a liquid substrate based on potato dextrose and olive oil in a 10L laboratory stirred tank bioreactor as well as in 15L solid state cultivation in a horizontal reactor in substrate based on beech sawdust and mineral media. In submerged cultivation, up to 17.0 gL~(-1) of dry fungal biomass was obtained in a fed batch process. From the mycelium biomass, 1.7 gL~(-1) of extracellular and 0.45 gL~(-1) of intracellular polysaccharides were isolated, which were mainly β-D-glucans. Polysaccharide fractions, tested in vitro for the induction of cytokine synthesis in primary cultures of human mononuclear cells from a buffy coat of healthy donors, induced 3.0-630 pg mL~(-1) of TNF-α, and 1.23-2.18 pg mL~(-1) of IFN-γ which is comparable to the activity of romurtide, a supporting drug in cancer therapy.