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AUTOLOGOUS TUMOR ORGANOID AND IMMUNE CELL CO-CULTURES AND METHODS OF USE AS PREDICTIVE MODELS FOR PANCREATIC CANCER TREATMENT
AUTOLOGOUS TUMOR ORGANOID AND IMMUNE CELL CO-CULTURES AND METHODS OF USE AS PREDICTIVE MODELS FOR PANCREATIC CANCER TREATMENT
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机译:自体肿瘤有机体和免疫细胞共培养和用途作为胰腺癌治疗的预测模型
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摘要
A method for preparing an autologous pancreatic tumor organoid and immune cell co-culture is provided, including: culturing pancreatic tumor cells obtained from a patient in a culture medium to provide a pancreatic tumor organoid and an organoid-conditioned medium; pulsing dendritic cells derived from the patient with a portion of the organoid-conditioned medium; contacting the pulsed dendritic cells with cytotoxic T lymphocytes (CTLs) obtained from the patient in the organoid-conditioned medium to activate the CTLs; isolating the activated CTLs; and co-culturing the pancreatic tumor organoid with the activated CTLs and myeloid derived suppressor cells (MDSCs) derived from the patient, to obtain an autologous pancreatic tumor organoid and immune cell co-culture that mimics the patient's pancreatic tumor microenvironment. Also provided are co-cultures obtained by the disclosed methods and methods of screening and determining whether a patient is likely to benefit from a candidate therapy.
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