Construction of next generation sequencing (NGS) library using competitive chain substitution

摘要

Problem to be solved: to provide a next generation DNA sequencing (NGS) library for all genomic sequencing, target directed resequencing, sequencing based screening assay, metagenomics, or any other application required for sample preparation for ngs.A method for preparing a target nucleic acid fragment for sequencingA. the first adapter sequence is connected to the 3 'end of the target nucleic acid fragment by a first ligaseFurther, the second adapter sequence is connected to the 5 'end of the target nucleic acid fragment by a second ligase.C. byThe first adapter sequence is adenylated at its 5 'endFurthermore, byThe first ligase comprises an adenylation deficient ATP dependent ligaseThe above method.No selection