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Gamma-globulin preparation for intravenous administration, process for production thereof and process for preparation of gamma-globulin of low anticomplementary activity
Gamma-globulin preparation for intravenous administration, process for production thereof and process for preparation of gamma-globulin of low anticomplementary activity
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机译:用于静脉内给药的γ-球蛋白制剂,其制备方法和制备低抗互补活性的γ-球蛋白的方法
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摘要
Conventional techniques of purifying gamma-globulin from human blood suffer from a variety of disadvantages, not least being problems associated with the removal of impurities of high anticomplementary activity. A gamma-globulin of an anticomplementary activity of lower than 20% (C'Hso value) in now prepared by forming a suspension of a pH of 7.0 to 9.0 of Cohn's Fraction II for said gamma-globulin in an aqueous solution of a monosaccharide, disaccharide or sugar alcohol, adding thereto dextran of an average molecular weight of 10,000 to 70,000 to produce an aqueous 2 to 10% wt./vol. solution of dextran and, after removing the thus formed precipitate, adding ammonium sulfate to the remaining mother liquor thereby to precipitate said gamma-globulin. A gamma-globulin preparation containing a gamma-globulin of such low anticomplementary activity is stabilized by specific amounts of L-arginine, L-lysine or a pharmaceutically acceptable salt thereof. The gamma-globulin and the preparation, which may be lyophilized, can be employed for intravenous administration to humans.
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机译:从人血中纯化γ-球蛋白的常规技术具有许多缺点,尤其是与去除具有高抗互补活性的杂质有关的问题。现在通过形成对所述γ-球蛋白的Cohn氏级分II的pH为7.0-9.0的悬浮液来制备抗互补活性低于20%(C'H so Sub>值)的γ-球蛋白。在单糖,二糖或糖醇的水溶液中,向其中加入平均分子量为10,000至70,000的右旋糖酐,以产生2至10%wt / vol的水溶液。右旋糖酐溶液,在除去由此形成的沉淀后,向剩余的母液中加入硫酸铵,从而沉淀所述γ-球蛋白。含有这样低的抗互补活性的γ-球蛋白的γ-球蛋白制剂通过特定量的L-精氨酸,L-赖氨酸或其药学上可接受的盐来稳定。 γ-球蛋白和可以冻干的制剂可以用于对人的静脉内给药。
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