Method of purification of fermentation liquids containing vitamin b12 and other corrinoids to the to prepare 13, vitamin b12 or vitamin b12 crystalline

摘要

The invention relates to a new process for purifying the fermentation liquids containing vitamin b and other corrinoids to the to prepare 13 of vitamin b, or vitamin b crystalline, in which the liquid of the fermentation ontraite containing the cells of the micro - organisms intact with the adsorption resins macroreticular nonionic of 10 - 8 a, 10 - 7 meters pore size, of at least 10 - 4 meters particle size of the diameter and of at least 200mg of specific surface and then, after disaggregation effected of the cells of a conventional manner, is fixed the corrinoids in a second step of adsorption on a resin of adsorption macroreticular similar or different, but also, nonionic, and it elue with an organic solvent miscible with water, optionally aqueous. / p & & p & after the first step of adsorption, it may also, if the desire, to separate the intact cells and to use the biomass thus obtained has the dry state immediately as an additive of the feeding stuff containing the vitamin b / p