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Probe for detecting chromosomal aberration induced by chromosomal translocation and method of detecting it
Probe for detecting chromosomal aberration induced by chromosomal translocation and method of detecting it
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机译:检测由染色体易位引起的染色体畸变的探针及其检测方法
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摘要
A probe for detecting a chromosomal aberration induced by chromosomal translocation, said probe comprising a DNA fragment or an RNA fragment containing 50 to 3,000 bases and having the ability to hybridize in a 2 x SSC solution at 40 oC with a DNA sequence existing between the second and third Hind III recognition sites on the bcr gene of a normal human chromosome 22, the Hind III sites being counted from the 3′-side of the first intron of the bcr gene, and a method of detecting a chromosomal aberration in leucocytes and/or lymphocytes, which comprises digesting a genome DNA of leucocytes and/or lymphocytes taken from a subject with a restriction endonuclease, subjecting the resulting DNA fragment to Southern hybridization using the aforesaid probe, and then detecting the resulting band.
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