human apolipoprotein a1 and its variant, expressed in escherichia coli.

摘要

An expression vector capable of expressing, in a transformed host, a protein which is capable of being detected by ELISA with anti-human apoAI antiserum and which has the formula (1) Met-X-Y (1) wherein X is a b ond, a carrier peptide sequence which comprises a sequence derived from the N-terminal amino acid residues of beta-galactosidase or a sequence comprising one or more IgG-binding domains of Protein A, or the pro sequence of human apoAI; and Y represents the sequence of human apoAI or a genetic variant thereof. Hosts transformed with such vectors produce the protein, which is useful in the treatment of atherosclerosis and cardiovascular diseases.