Shuttle vector, utilizing the E. coli asparagine synthetase gene, and capable of dominant transfection and amplification in animal cells

摘要

A shuttle vector capable of dominant transfection of both animal and bacterial cells and of amplification in animal cells has been constructed. This vector contains the cloned bacterial gene coding for asparagine synthetase; it is shown that animal cells bearing the vector can be selected for with a drug which inhibits the animal but not the bacterial asparagine synthetase. The vector and any linked genes can then be amplified using a drug which inhibits the bacterial synthetase. This vector is useful in the production of large quantities of specific biological products by animal cells. It may also be useful for the genetic transformation of plants.