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DETECTION OF HUMAN PAPILLOMAVIRUS DNA AND ITS EXPRESSION IN CERVICAL SMEARS
DETECTION OF HUMAN PAPILLOMAVIRUS DNA AND ITS EXPRESSION IN CERVICAL SMEARS
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机译:人乳头瘤病毒DNA的检测及其在宫颈涂片中的表达
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摘要
The invention relates to the direct detection of human papillomavirus DNA. The recently developed polymerase chain reaction has been modified in order to improve sensitivity and specificity. It is possible, by choosing suitable oligonucleotide primers (amplimers) and reaction temperatures, for individual HPV genes to be identified among the total cell 4 DNA and amplified to such an extent that non-isotopic detection is possible. In addition, the use of reverse transcription allows deliberate amplification of spliced mRNA and thus an indication of premalignant or malignant states or lesions.
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