RECOMBINANT PLASMID DNA HIV 24-5, ENCODING FUSED PROTEIN OF HEPATITIS B VIRUS CORE-ANTIGEN AND ENVELOPE PROTEIN OF HUMAN IMMUNODEFICIENCY VIRUS, AND STRAIN OF BACTERIA ESCHERICHIA COLI - A PRODUCER OF FUSED PROTEIN OF HEPATITIS B VIRUS CORE-ANTIGEN AND ENVELOPE PROTEIN OF HUMAN IMMUNIDEFICIENCY VIRUS

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 to the microbiological and medical industry and biotechnology. purpose u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 u043fu043eu043bu0443u0447u0435u043du0438u0435u0431u0435u043bu043au0430 with immune boosting gene activity in the u044du043du0442u0438u0433u0435u043du0430u0438 virus hepatitis b and virus protein shell u0438u043cu043cu0443u043du043eu0438u0437u043eu0431u0440u0435u0442u0435u043du0438u0435 from u043du043eu0441u0438u0442u0441u00a0 to the microbiological and medical industry and biotechnology.purpose to obtain u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 protein with u0438u043cu043cu0443u043du043eu0433u0435u043du043du043eu0439 activity in hepatitis b virus antigen and protein of the human immunodeficiency virus man as well. designed u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u00a0 u043fu043bu0430u0437u043cu0438u0434u043du0430u00a0 dna u0441u043eu0441u0442u043eu00a0u0449u0430u00a0 the following elements: the lack of rights.the invention u043fu043eu0437u0432u043eu043bu00a0u0435u0442 get u0441u043bu0438u0442u044bu0439 protein u043du0432u0441u0430u0434, u0441u043eu0441u0442u043eu00a0u0449u0438u0439 of 114 n end amino acids in virus hepatitis b antigen (ddp) and full u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du043eu0439 u043fu043eu0441u043bu0435u0434u043eu0432u0430 u044024) protein of human immunodeficiency virus (hiv). these proteins possess immunological activity as u043du0432u0441u0430u0434, and protein u044024 hiv.in addition to 144 amino acids u043du0432u0441u0430u0434 recombinant protein u043du0432u0441u0430u0434 AHIVp24 contains the following sequences of gag gene of hiv: 13 amino acid protein u044017, 231 amino acids u044024 and 74 amino acid u044015.protein synthesis u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u00a0 encodes u043fu043bu0430u0437u043cu0438u0434u0430 pHIV 24 - 5, u043au043eu0442u043eu0440u0430u00a0 consists of dna plasmids u0440u043du0432u0441u0431 with gene u043du0432u0441u0430u0434 with optimized site initiation u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 and with u0442u0435u0440u043cu0438u043du0438u0440u0443u044eu0449u0438u043c u043au043eu0434u043eu043du043eu043c to 144. u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u0435 gene, and a fragment of the genome of hiv, cloned in u043fu043bu0430u0437u043cu0438u0434u0435 u0440u0432u043du044e and the consistency between sites Pvull and restriction in the Bglll gag. the size of the plasmid 7900 sukhoy., u043cu043eu043b.u043c. 5.06 mda gene u044bu0430 provides resistance plasmids to u0430u043cu043fu0438u0446u0438u043bu043bu0438u043du0443. the gene u043du0432u0441u0430u0434 dr. HIVp24 u043du0430u0445u043eu0434u0438u0442u0441u00a0 under control of tandem u043fu0440u043eu043cu043eu0442u043eu0440u043eu0432 u0442u0440u0438u043fu0442u043eu0444u0430u043du043eu0432u043eu0433u043e u043eu043fu0435u0440u043eu043du0430. 2 p.s - lu, 2 table u0440u043du0432u04415 dna plasmids, u043au043eu0442u043eu0440u0430u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 u0440u043du0432u0441u0437 derivative plasmids with gene u043du0432u0441u0430u0434 with optimized site initiation u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438, but with u0443u043au043eu0440u043eu0447u0435u043du043du044bu043c the u043du0430u0441u0430u0434 deprived of 39 n end amino acids and with a unique restriction site EcoRI no. 144. u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u0435 gene u043fu0440u043eu0442u00a0u0436u0435u043du043du043eu0441u0442u044cu044e 6700 sukhoy., a fragment of the genome of human immunodeficiency virus (hiv), cloned in u043fu043bu0430u0437u043cu0438u0434u0435 u0440u0432u043du044e, the u043fu043eu0441u043bu0435XI sl u043fu0448u0434 yu to yu