RECOMBINANT PLASMID DNA HIV 41-22, ENCODING CORE-ANTIGEN OF HEPATITIS B VIRUS WITH EXPOSED ON ITS SURFACE PROTEIN FRAGMENT @@41 OF HUMAN IMMUNODEFICIENCY VIRUS, METHOD FOR ITS CONSTRUCTION AND THE STRAIN OF BACTERIA ESCHERICHIA COLI A PRODUCER OF HEPATITIS B VIRUS CORE-ANTIGEN WITH EXPOSED ON ITS SURFACE PROTEIN FRAGMENT @@41

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 to the microbiological and medical industry and biotechnology. purpose to obtain u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 structures with u0438u043cu043cu0443u043du043eu0433u0435u043du043du043eu0439 activity in hepatitis b virus antigen and protein u0434u044041 human immunodeficiency virus.the invention u043fu043eu0437u0432u043eu043bu00a0u0435u0442 get protein u043au0430u043fu0441u0438u0434u043du044bu0435 structure u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u044eu0449u0438u0435 a cor - antigen hepatitis b virus with u044du043au0441u043fu043eu043du0438u0440u043eu0432u0430u043du043du044bu043c on its surface u0437u043fu0438u0442u043eu043fu043eu043c the human immunodeficiency virus (hiv) u043du043cu0434u044041 (78 - 129) and non immunological activity as kor - antigen, and protein u0434u044041.the synthesis of such structures u0440u0435u043au043eu043cu0431u0438u043du0430u043cu0442u043du0430u00a0 encodes u043fu043bu0430u0437u043cu0438 - yes pHIV41 - 22, u043au043eu0442u043eu0440u0430u00a0 contains gene u043du0432u0441u0430u0434 with optimized site initiation and u043fu043eu043bu0438u043bu0438u043du043au0435u0440u043du043eu0439 in u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 rate of 144. u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u0435 where cloned fragment sequence between restriction sites u0437u0430u0438u0437u0430 and Hindll, and encrypting the u0434u044041 (78 - 129) u043fu0440u043eu0442u00a0u0436u0435u043du043du043eu0441u0442u044cu044e 154, para. o - a fragment of a protein u0434u044041 with 78th on 129 different amino acids.the size of the plasmid with sukhoy. the gene u044bu0430 provides resistance to u0430u043cu043fu0438u0446u0438u043bu043bu0438u043du0443. the gene HBcAg - HIV41 (78 - 129) u043du0430u0445u043eu0434u0438u0442u0441u00a0 controlled tan dema u043fu0440u043eu043cu043eu0442u043eu0440u043eu0432 Ptrp and provides a synthesis of the target product. the strain is a producer has an exit end product 3.3% of total protein. coli. 3 boeing. s - lu, 2 table. sk n sh, yu q on this.