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DNA EXTRACTION REAGENT FROM LIVING THING SAMPLE, DNA EXTRACTION METHOD USING REAGENT, AND DNA EXTRACTION KIT
DNA EXTRACTION REAGENT FROM LIVING THING SAMPLE, DNA EXTRACTION METHOD USING REAGENT, AND DNA EXTRACTION KIT
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机译:生活样品中的DNA提取试剂,使用试剂的DNA提取方法以及DNA提取试剂盒
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摘要
PURPOSE: To extract DNA with a high yield and a high purity with a simple operation quickly by allowing an aqueous solution including at least one type selected from a group consisting of protein denaturant and a reducer and then a surface-active agent and chelator to be made of a protein dissolution agent. ;CONSTITUTION: The title DNA extraction reagent is an aqueous solution including a protein denaturant as an essential ingredient. A chaotropic agent is desirable as the protein modification agent. Further, the reagent includes at least one type selected from a group consisting of a reducer, a surface-active agent, and chelator. Thiol reducer which can cut disulfide coupling, one which does not cause settlement in the aqueous solution with a reagent composition, and one which has a capacity for chelating divalent metal ion are desirable as the reducer, the surface-active agent, and chelator, respectively. The reagent is used with its pH being from weak acid to weak alkali.;COPYRIGHT: (C)1994,JPO
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